pgFARM (paired guide RNAs for alternative exon removal)

We use paired guide RNAs (pgRNAs) for isoform-level genetic screens. Resources for using pgRNAs include:

  • protocol for cloning single pgRNAs
  • protocol for cloning and sequence-validating pgRNA libraries
  • “pgPoison” library (pgRNA library targeting poison exons and upstream constitutive exons)

    Please cite Thomas et al, Nature Genetics, 2020 if you use pgFARM.

    • Plasmids

    Many of our other plasmids and expression constructs are available from our Addgene page.